Composite

Part:BBa_J40001

Designed by: Horia Vulpe   Group: iGEM06_McGill   (2006-10-10)

LacI (LVA) + ECFP (LVA) under luxpR promoter (LuxR/HSL regulated)

Polycistronic LacI and ECFP proteins with degradation tags regulated by luxpR promoter (expression up-regulated by LuxR/HSL complex). Used for quorum sensing stabilization of repressilator system.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1228
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None